| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8603ee14f13e9c3d3f6d5858bf4f3bf |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate |
2007-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae32af1bfb3ea751d5ea053269577b48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b74f66e6e8a053a7cb3c001deeb7f60a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27e77bd0fbd0b64ed123d58655ff4661
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af01bb1357c916e198029b2c52098ced
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_decb8dd7bcf0148bd7fb632e66cc2030
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7704bb66ba28a8bf417b45d4cb3fae6 |
| publicationDate |
2010-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2010120089-A1 |
| titleOfInvention |
A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same |
| abstract |
Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015113494-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9499584-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011117643-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8426570-B2 |
| priorityDate |
2006-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |