http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010120037-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa2ea32e3cda1d0118f53c48b589cfed http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2270201a16c788406ac207571d31f862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ce44b18222b06740e68821a60f5a31e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 |
| filingDate | 2009-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb71b2427cd919aa1e7fc674f2595312 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8870c8e85509eae3d1579d90f99411d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c75d6f669ad96a77777a905e3a31d749 |
| publicationDate | 2010-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2010120037-A1 |
| titleOfInvention | Method for isolating and culturing unculturable microorganisms |
| abstract | The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained. |
| priorityDate | 2002-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.