| abstract |
Methods for analyzing a mixture of polynucleotides are provided. In some embodiments, a plurality of detection primers comprising target-specific segments complementary to regions in said polynucleotides are employed to generate a library of 3′-end labeled detection primers after hybridization with said mixture of polynucleotides, the detection primers optionally including unique barcode sequences. The labeled detection primers can be enriched and subjected to microarray and/or sequencing analysis. The subject methods can be used, for example, in comparative genomic hybridization, gene expression analysis, methylation analysis, copy-number variation, genome partitioning and other applications. Also provided are kits for use in practicing the subject methods. |