| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3d914ec25b270306d328c6846b59165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_09d1b6a0165a2cb5c937487c5a74c49f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46a74bd99823f164bb18641d371fcc4a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2008-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a10d17fce495a5f38ee4369af3976190
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0bc92ea6eb80e896debe4d40f37edac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49b920a812bb9271ac2a22b0b27b7d97 |
| publicationDate |
2010-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2010075307-A1 |
| titleOfInvention |
Analyte detection assays |
| abstract |
The present invention provides methods, kits and compositions for the detection of an analyte. In the methods of the invention, a binding molecule coupled to a first polymerase is incubated with a modified polynucleotide template to form a copy of the modified polynucleotide template without any modified nucleotides. The unmodified copy is detected in a second amplification/primer extension reaction using a second polymerase that is unable to amplify the modified polynucleotide template. Detection of the unmodified copy is indicative of the presence and/or amount of the analyte in the sample. In place of the binding molecule coupled to a first polymerase, a pair of analyte-specific probes can also be used. The first analyte specific probe comprises a first binding moiety and a first portion of a first polymerase and the second analyte specific probe comprises a second binding moiety and a second portion of the first polymerase. When the binding moieties are bound to the analyte the first and second portions of the polymerase interact to form a functional polymerase complex that is used to form a copy of the modified polynucleotide template without any modified nucleotides. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10000796-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111398576-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11634753-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015368625-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10982263-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10718772-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11168350-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9909167-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10370698-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2023002809-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11299770-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009298088-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10017808-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011223585-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10006082-B2 |
| priorityDate |
2007-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |