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publicationDate 2010-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2010047855-A1
titleOfInvention Method for determining the quantity of microbiological objects during cultivation thereof
abstract A method for determining the quantity of microbiological objects during the cultivation thereof comprises determining a modification of studied microorganism cells' population, measuring the morphological composition thereof by determining the cells' size distribution in a liquid medium according to intensity changes of the light dispersed thereby. The method comprises probing a liquid flow by monochromatic coherent light, recording signals relating to the interaction between the probing radiation and the cells by measuring amplitudes and durations of impulses of the light dispersed by medium particles, plotting functions, according to measurement results, as two-dimensional distributions of normalized values of the amplitudes and durations in the form of statistic parameters of the dispersed light intensity. According to the functions, the size distribution of the cells and decomposition products of the liquid nutrient medium during the cultivation thereof are obtained. The invention can be applied for medical diagnostics and for control of biotechnological processes.
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