Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5ad8562c578b061df7a2c3aee9d0e24 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
filingDate |
2008-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca06a642df4297326ea089bd186330de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c35d28f708c11bd3dbdb0f97f8ef71db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f2b969bad6334f3d53637f772ec02d0 |
publicationDate |
2010-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2010028950-A1 |
titleOfInvention |
Method for the immobilization of biologically active polypeptides by using maltose binding protein |
abstract |
The present invention relates to a method for immobilization of a biologically active polypeptide using maltose binding protein (MBP) and a biologically active solid substrate on which a biologically active polypeptide is immobilized by the above method. More particularly, the present invention relates to a method for immobilization of a biologically active polypeptide comprising the following steps; 1) preparing a fusion protein by linking a biologically active polypeptide to carboxyl terminal of maltose binding protein (MBP); and 2) immobilizing the fusion protein on the hydrophobic surface by physical adsorption of amino terminal containing hydrophobic domain exposed on the surface of maltose binding protein on the hydrophobic surface of a solid substrate, and a biologically active solid substrate on which a biologically active polypeptide is immobilized by the said method. |
priorityDate |
2008-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |