http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010009434-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b99a69eabf234c8bcf6481d32ea23d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0443ea9c8ede12257dd0e402dc1bf397 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb6d141797e7037d391bb615ddf5080f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aeef3940e47351e7990fa4172dd8487d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 |
| filingDate | 2006-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bba88f05e2bd3233cf028038bf3292ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70fc9a568e742333dc823c418834a838 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ea91b006053cbcc019105ecdeea0444 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f03abb36b1095535008ff2c123c8029e |
| publicationDate | 2010-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2010009434-A1 |
| titleOfInvention | Method for the Selective Enrichment of Double-Stranded Dna from Nucleic Acid Mixtures |
| abstract | The invention relates to a method for stripping undesired nucleic acid components from double-stranded DNA, in particular, super-coiled plasmid DNA. The method according to the invention is characterised by the steps: (a) provision of a mixture containing completely and/or partly double-stranded nucleic acids and optionally single-stranded nucleic acids; (b) resuspension of the mixture from step (a) in an aqueous, low-molarity buffer system with low ionic strength and low buffer effect; (c) adjusting conditions in the mixture from step (b), under which the completely and/or partly double-stranded nucleic acids are denatured; (d) further addition of buffer and a polymer component to the mixture from step (c); (e) incubation of the mixture from step (d) for a time which is sufficient for the formation of an aqueous two-phase system with an upper and lower phase; and (f) removal of the upper phase containing the single-strand nucleic acid and collection of the double-strand nucleic acid from the lower phase. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012080968-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017273677-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/PT-105438-B |
| priorityDate | 2005-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.