http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010003686-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8def5adebe7616f8937efbbba81e4ce6 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-20 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-635 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
filingDate | 2007-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52bf88d15aa428b07ea47bde6ab72e9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7016f1b842138c6021e991db3c1e9b3a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8ad5230602103bbf0aced9ac30266bd |
publicationDate | 2010-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2010003686-A1 |
titleOfInvention | Process for producing tetracycline inducible gene expressing cell line and conditional gene knockout cell line, and uses thereof |
abstract | It is intended to provide a method of screening a so-called Tc inducible gene expressing cell line, in which the expression of a gene of interest is regulated depending on the presence/absence of a Tc compound regardless of the type of cell, easily with high efficiency. A gene-of-interest expression vector for transfecting a gene of interest into the genome of a host cell and a TA expression vector that expresses a transactivator that is switched to be bound or not to be bound to a tet operator sequence depending on the presence or absence of tetracycline are prepared. The gene-of-interest expression vector is a vector with a bicistronic regulatory sequence arranged downstream of the tet operator sequence and promoter sequence and between a gene-of-interest coding sequence and a selection marker coding sequence. These vectors are transfected into a host cell, and cell lines in which the selection marker of the gene-of-interest expression vector is expressed are selected. Thus, inducible gene expressing cell lines can be obtained in which the expression of a gene of interest can be controlled with Tc. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109456991-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011027621-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2431386-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012035088-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9181556-B2 |
priorityDate | 2006-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 842.