| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_700ff9a01db2075489cb7ed87392dc28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41a639aa11b9b2f8063a3e6b8b22b557 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6454
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P43-00 |
| filingDate |
2008-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_205db7a084a6ed9dc127074d3f076f45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c043122b1a22180f024d5fe282da1638
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_624da27805cbf5f3b12060aed8d5c9e0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2dfd17f55335d3a3d3850f918fe02056 |
| publicationDate |
2009-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2009304669-A1 |
| titleOfInvention |
Preparative purification process for human furin |
| abstract |
Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC™ resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC™ chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8877473-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013004587-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012167271-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9662376-B2 |
| priorityDate |
2007-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |