http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009298133-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62fb73284de3d978128c7d0e141e8442 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e44ddd3a782e5fd96a7d22b7b716f706 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f82669294f169e03e642037787712ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f6a66740b9a58a1772e91994efe861e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2005-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_880276237b5e2fff47e9c52e286172b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aff60e6d0a20086e39c03341d6d43df8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70c1fd0d46b61fed2751f32748cfdfbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80213de87d72572bb70496d0db19332c |
| publicationDate | 2009-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2009298133-A1 |
| titleOfInvention | De novo enzymatic production of nucleic acid molecules |
| abstract | The present invention is related to a method for the manufacture of a nucleic acid molecule comprising the following steps: a) providing a first at least partially double-stranded oligonucleotide, whereby the oligonucleotide comprises a first and a second single-stranded overhang, b) providing a second at least partially double-stranded oligonucleotide, whereby the oligonucleotide comprises a recognition site for a first type IIS restriction enzyme which cuts outside its recognition site, a modification allowing the oligonucleotide to be coupled to a surface and a single-stranded overhang, c) ligating the first oligonucleotide and the second oligonucleotide via the first single-stranded overhang of the first oligonucleotide and the single-stranded overhang of the second oligonucleotide, generating a first ligation product, whereby the first ligation product comprises a single-stranded overhang essentially corresponding to the second single-stranded overhang of the first oligonucleotide, d) cutting the first ligation product with the first type II restriction enzyme thus releasing an elongated first at least partially double-stranded oligonucleotide having a first and a second single-stranded overhang, whereby the first single-stranded overhang is generated through the cutting of the restriction enzyme and whereby the second single-stranded overhang corresponds essentially to the second single-stranded overhang of the first at least partially double-stranded oligonucleotide, preferably the at least partially double-stranded oligonucleotide of step (a), and a truncated second at least partially double-stranded oligonucleotide; e) immobilising the truncated second at least partially double stranded oligonucleotide of step d), the unreacted second at least partially double-stranded oligonucleotide and/or the uncut first ligation product via the modification to a surface; f) optionally repeating steps a) to e), whereby the elongated first at least partially double-stranded oligonucleotide of step d) serves as the first at least partially double-stranded oligonucleotide in step a). |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015176070-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9187777-B2 |
| priorityDate | 2004-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.