| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e15c29ea789bdea9e355917f3e007d72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88b765f60df1dc351b13ce12c49441ea |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
2006-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_683e4084617a3940ed7492dafe740f44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1445ce8ae32867b9878da922b04b55a4 |
| publicationDate |
2009-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2009269801-A1 |
| titleOfInvention |
Vector to Induce Expression of Recombinant Proteins under Anoxic or Microaerobic Conditions |
| abstract |
A protein expression vector that expresses large quantities of recombinant proteins under anoxic or microaerobic conditions by inducing expression with nitrate. The vector backbone is pUC19 and protein expression is driven by the E. coli flavohemoglobin promoter, which is inducible by nitrate, nitrite, or nitric oxide under conditions of low oxygen. The Nde1 site of pUC19 has been destroyed by filling in with Klenow fragment and religating the vector. An Nde1 site in the promoter provides an in-frame start methionine and a standard polylinker is available for ease of subcloning. The vector is named pANX for Plasmid ANaerobic eXpression. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012270297-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114854809-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10927382-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018164748-A1 |
| priorityDate |
2005-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |