http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009228999-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fba5ef972720f17c5f58279c41bebdf5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2830-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2008-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f455205413e74105aa4083bf1d9934b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_127938902f264e172f51150ea85dc3d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0f353cc35804163f9663d0d9f173c2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05cd98b68a42c7c70a1c43f3fdc231c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_273096cbea781356a379e8dc34a2db64 |
| publicationDate | 2009-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2009228999-A1 |
| titleOfInvention | Process of Making Transgenic Mammals That Produce Exogenous Proteins in Milk and Transgenic Mammals Produced Thereby |
| abstract | The invention relates to a non-human transgenic mammal that is useful for the production of a protein of interest that may be toxic to the mammal. The mammal is characterized by the fact that it is transgenic for the production in its milk of an inactive form of the protein of interest, preferably recombinant human insulin. It is not possible to produce recombinant human insulin in transgenic mammals since this molecule has a certain degree of biological activity in the mammals and could be toxic to the mammal. Thus, the invention involves cloning a genetic construct comprising a sequence encoding a modified human insulin precursor under the control of a beta casein promoter in an expression vector. It also involves transfecting the expression plasmid into fetal bovine somatic cells, such as fibroblasts, and enucleating bovine oocytes by nuclear transfer to generate transgenic embryos. The invention gives rise to transgenic bovine that will be able to produce a modified human insulin precursor in their mammary glands. Afterwards, the milk of these transgenic mammals can be collected, the modified human insulin precursor can be converted in vitro into recombinant human insulin, and the recombinant human insulin can be purified to homogeneity as a pure biopharmaceutical product. |
| priorityDate | 2007-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 460.