| abstract |
The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X 1 -cysteine sequence, wherein X 1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X 2 -X 3 , wherein X 2 is any amino acid, X 3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X 1 -cysteine sequence. |