http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009162860-A1

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filingDate 2008-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38e3508171263aac9e6fc4896959d761
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publicationDate 2009-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2009162860-A1
titleOfInvention Method of nucleic acid sequence detection and nucleic acid sequence detection substrate
abstract According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
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