| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f09962a448c78a6faea3475a067eaf44 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate |
2007-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a0f2adf33ec28bd87c5e2c17c1b774c |
| publicationDate |
2009-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2009156431-A1 |
| titleOfInvention |
Methods for Nucleic Acid Mapping and Identification of Fine Structural Variations in Nucleic Acids |
| abstract |
An in vitro, extracellular method of juxtaposing sequence tags (GVTs) where two constituent members of a tag pair (GVT-pair) are unique positional markers of a defined separation distance and/or are markers of nucleic acid positions that demarcate adjacent cleavage sites for one or more different restriction endonucleases along the length of a plurality of target nucleic acid molecules, the method comprising: Fragmenting the target nucleic acid molecule to form target DNA insert; ligating a DNA adaptor having one or more restriction endonuclease recognition sites to both ends of a fragmented target DNA insert and the ligation of the adaptor-ligated target DNA insert to a DNA backbone to create a circular molecule; digesting the adaptor using a restriction endonuclease at the recognition site to cleave the target DNA insert at a defined distance from each end thereof to create two sequence tags (GVTs) comprising terminal sequences of the target DNA insert that are attached to the linear DNA backbone; and recircularizing the linear DNA backbone with the attached GVTs to obtain a circular DNA molecule including a GVT pair having two juxtaposed GVTs; GVT-pair DNA is recovered by nucleic acid amplification. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10494670-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9857328-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9859394-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10429342-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10006910-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10429381-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10607989-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9309560-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10811539-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10190164-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9822395-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9540637-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009239764-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9932636-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10020300-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450608-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9618474-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9074244-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9657291-B2 |
| priorityDate |
2007-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |