http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009123917-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_51a0c49e5390c69aa92b87c3a892344c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_178db715e3706766149e1b26f99ecb41 |
| publicationDate | 2009-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2009123917-A1 |
| titleOfInvention | Methods for quantifying microrna precursors |
| abstract | Provided herein is a sensitive, high throughput, real-time PCR assay to monitor the expression of miRNA precursors. Gene specific primers and reverse transcriptase were used to convert the primary precursors and pre-miRNAs to cDNA. The expression of 23 miRNA precursors in six human cancer cell lines was assayed using the PCR assay. The miRNA precursors accumulated to different levels when compared to each other or when a single precursor is compared in the various cell lines. The precursor expression profile of three miRNAs determined by the PCR assay was identical to the mature miRNA expression profile determined by Northern blotting. We propose that the PCR assay may be scaled up to include all of the 150+ known human miRNA genes and can easily be adaptable to other organisms such as plants, C. elegans and Drosophila. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008220423-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012220468-A1 |
| priorityDate | 2005-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.