Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e70c0aeade92d7571d955d3b90ea06e |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-20 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 |
filingDate |
2008-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acd81e22f7600326554d57dc6c0ecb0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a8c08552946037b2b224b662ca0acde http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3bdf9f05815b4c7c334663166141e52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f128944ad9cbc9299ab1616719c657e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f18807fcf8caba35dd9d99cd391996c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10aed29bc2e73d8b19ef8c74f5c4116f |
publicationDate |
2009-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2009117609-A1 |
titleOfInvention |
Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins |
abstract |
A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011174064-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010038228-A3 |
priorityDate |
2007-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |