abstract |
A method for analyzing a pathological deviation of at least one white blood cell population from a normal level in an un-lysed blood sample, comprising the steps of counting, with a flow cytometer, a number, n 1 , of white blood cells expressing a first marker; a number, n 2 , of white blood cells expressing a second marker, and a number, n 3 , of white blood cells expressing a third marker but not the first marker; and comparing the sum (n 1 +n 2 +n 3 ) with a reference value. The sum (n 1 +n 2 +n 3 ) may represent the number of lymphocytes. The first, second and third markers may be chosen from the group consisting of CD56, CD3 and CD19. |