| abstract |
The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or enrichment of protein(s) from a sample, followed by separation/identification thereof. In general terms, a microfluidic reverse affinity-blot device is a closed system of interconnected components that is comprised of defined points of entry and exit, wherein the interconnected components include a capture region upstream from a protein separation region and subsequent detection region. Methods of use are also described. |