| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e8f9e7a1094964e5ce47ad57c20018d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6046d8af2c5981f5a981b9b0ec25ef45 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2008-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40dd366a1f1e601498feb728eb406c93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cf3da9b2b61532049d9f00e84fac842 |
| publicationDate |
2009-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2009087858-A1 |
| titleOfInvention |
Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs) |
| abstract |
The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011117559-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10781486-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010112573-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9657346-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011060014-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9068222-B2 |
| priorityDate |
2004-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |