http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009081656-A1

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filingDate 2008-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd29927d42ea4bc1aed5cb3ec4b5f47f
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publicationDate 2009-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2009081656-A1
titleOfInvention Method for detecting a bacterial pathogen
abstract A method is provided for detecting a bacterial pathogen in a sample. One step of the method includes obtaining a sample and then subjecting the sample to nested PCR. The nested PCR is conducted in the presence of at least two outer oligonucleotide primers complementary to a target nucleotide sequence of the bacterial pathogen so that a first amplified product is produced. The target nucleotide sequence includes at least a portion of a 16S-23S ribosomal RNA sequence. The first amplified product is subjected to the nested PCR in the presence of at least two inner oligonucleotide primers complementary to the nucleotide sequence of the first amplified product so that a second amplified product is obtained. Detection of the second amplified product indicates the presence of the bacterial pathogen in the sample.
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priorityDate 2007-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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