http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009062515-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_268be9afa00cf55b5aa72b1612151ecb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 |
| filingDate | 2008-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_216800c13bf3ab41788375e6c897230c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0acfc1e6d336e1337dda86055f929ba8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb9029e05fc57b84adfe5ec31953de95 |
| publicationDate | 2009-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2009062515-A1 |
| titleOfInvention | Method for purifying bioactive substances |
| abstract | A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution. |
| priorityDate | 2007-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 223.