Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ddb5193471ae29da9c0e70c0a51595c |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04 |
filingDate |
2006-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bce3e0dbcd4ecba0766b8cdc3828e23 |
publicationDate |
2009-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2009053761-A1 |
titleOfInvention |
Polypeptide Mutagenesis Method |
abstract |
There is provided a method for altering the amino acid sequence of a target polypeptide by altering a target DNA sequence which encodes that polypeptide, the method comprising the step of introducing a transposon into the target DNA sequence, in which the transposon comprises a first restriction enzyme recognition sequence towards each of its termini, the recognition sequence not being present in the remainder of the transposon, or in the target DNA sequence, or in a construct comprising the target DNA sequence, the first restriction enzyme recognition sequence being recognised by a first restriction enzyme which is an outside cutter and being positioned such that the first restriction enzyme has a DNA cleavage site positioned beyond the end of the terminus of the transposon. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009264313-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014039662-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022333096-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8357638-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015337290-A1 |
priorityDate |
2005-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |