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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e43d10f04beb103f6a77be442316acc2
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publicationDate 2009-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2009042236-A1
titleOfInvention Method of identifying hydrogen evolving diazotrophic bacteria
abstract MohC is required for expression of uptake hydrogenase in A. vinelandii and inactivation of this gene results in H 2 evolution via nitrogenase 3. Since MohC − mutants are tungsten tolerant under nitrogen-fixing conditions, tungsten can be used to select spontaneous tungsten-tolerant mutants of nitrogen-fixing bacteria that are genetically uncharacterized. A major advantage of producing H 2 via nitrogenase 3 is that carbon substrates used to culture the nitrogen-fixing bacteria harboring this nitrogenase are derived from plant sources.
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