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filingDate 2008-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2009-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2009029383-A1
titleOfInvention Polynucleotide Sequencing Method
abstract The subject invention pertains to a method for determining the sequence of a polynucleotide comprising the steps of (i) contacting a polynucleotide processive enzyme immobilised in a fixed position, with a target polynucleotide under conditions sufficient to induce enzyme activity; (ii) detecting an effect consequent on the interaction of the enzyme and polynucleotide, wherein the effect is detected by measurement of a non-linear optical signal or a linear signal coupled to a non-linear signal.
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