Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_255ea743d5f234e70fb6a537572bcaa2 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-98 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5011 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 |
filingDate |
2008-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2f50c727e2d60fa1ff59b51acd1bae2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb19580a30a824ea460998820483741d |
publicationDate |
2008-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2008286815-A1 |
titleOfInvention |
Spectrophotometric assay for human histone deacetylase 8 |
abstract |
The present invention relates to the development of a novel assay for determining the activity of human histone deactylase 8 (HDAC8). As shown in FIG. 3, thioactyl-lysine-containing peptide is used as the substrate for the HDAC8-catalyzed dethioacetylation reaction. Thioacetate, which is formed during this reaction, is subsequently reacted with Ellman's reagent, 5,5′-dithiobis(2-nitrobenzoate), (DTNB) with a quantitative formation of 2-nitro-5-thiobenzoate (TNB). The concentration of thioacetate formed during the conversion reaction can be quantified by measuring the absorbance of TNB at 412 nm. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112526051-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104198404-A |
priorityDate |
2007-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |