| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b0856636769205433ade58205184b8a |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2007-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43652d83ad0594b96e8ddc3f98d30955
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aefbe8acce9fde6b813c35335735af6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f112c793b60f84ee0b25d758044e697 |
| publicationDate |
2008-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2008193940-A1 |
| titleOfInvention |
Systems and methods for detecting nucleic acids |
| abstract |
A method and kit for detecting a target nucleic acid in a sample is described. The sample to be analyzed may include a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising exonuclease activity that can cleave the hybridized hybridization probe to thereby generate a labeled probe fragment. At least one portion of the hybridization probe hybridizes to another portion of the hybridization probe to thereby form a folded structure. The method can involve melting the sample, reducing the temperature of the sample to allow primer and probe to each hybridize to at least a portion of single stranded target nucleic acid in the sample, elongating the primer and releasing the labeled probe fragment. The sample can be contacted with a solid support comprising surface bound capture probes which hybridize to the labeled probe fragments. The label can then be detected. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10519489-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10280453-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012150835-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10731203-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012150749-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9650665-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11078525-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11306349-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8703653-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013133561-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015072887-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11447814-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11401546-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016258007-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9868980-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9540681-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9850524-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012214686-A1 |
| priorityDate |
2006-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |