http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008113421-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e071d18fea054bf8d1420d483e78ed1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-386 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
| filingDate | 2006-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_998c84dcef9a9542e1cfe79690bbf59c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e51cc0246af93be45d241afc1687a449 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af26ba1eb111ad501b37835558686992 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c01ae8880f0be6a7cca12bcfb99ad75f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bc8077bc4c46f3dbca5eed13c44fd00 |
| publicationDate | 2008-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2008113421-A1 |
| titleOfInvention | Stabilizing proteins against denaturation and inactivation by charged detergents using chemical modifications, including modifications that increase net charge |
| abstract | The present invention generally relates to enzymes and other proteins resistant to denaturation, and techniques for making and using the same. In one aspect, lysine and/or other charged residues within an enzyme are reacted in some fashion, which can render the enzyme more resistant to denaturation. For example, the lysine residue may be neutralized by acetylating the residue, for instance, by exposure to acetic anhydride. In some aspects, the enzyme, after reaction, may be relatively resistant to degradation when placed in a harsh environment, for example, when exposed to sodium dodecyl sulfate at a concentration of at least about 2.5 mM in Tris-Gly buffer. The enzyme may still be susceptible to denaturation in some cases, but at a much slower rate (e.g., the denaturation time constant may be higher). Other aspects of the invention are directed to enzymes prepared in such fashion, methods of promoting or using such enzymes, kits involving such enzymes, and the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014012181-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015103545-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10584150-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9546208-B2 |
| priorityDate | 2006-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 275.