abstract |
Monovalent Fab labeling reagents are conjugated to spectrally-resolvable fluorescent dyes. Each of these reagents are incubated with a patient sample comprising antibodies, to allow the monovalent Fab fragments to bind and label the antibodies. The patient samples are then used directly to contact and stain antigen, e.g. an antigen microarray, histologic section, tissue microarrays, cells, etc. |