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filingDate 2007-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2008-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2008085539-A1
titleOfInvention Methods of generation and use of in vivo biotinylated recombinant antibodies
abstract The present invention provides compositions and methods for generating in vivo biotinylated, secreted recombinant polypeptides comprising recombinantly co-expressing in a yeast cell (a) a secreted recombinant polypeptide comprising a biotin accepting site; and (b) a biotin ligase fused to the golgi localization domain so that the biotin ligase is localized to the golgi of the yeast cell where it acts on the biotin-accepting site of the secreted polypeptide as it transits the secretory system.
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