Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_144f594513aec7b42679f432e92531e8 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
filingDate |
2007-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eed6514edaa55f91c80366f6082a6d44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00121e51f5404d3fadcea1d4a4a7627f |
publicationDate |
2008-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2008076912-A1 |
titleOfInvention |
Nucleic acid purification method |
abstract |
The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes providing the anion exchanger in a packed column, wherein the column is packed using a salt solution containing an antimicrobial agent. In addition, the salt solution has a salt concentration similar to that of the lysate, such that the column does not need equilibration prior to sample loading. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9856459-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10023860-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013017552-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10801042-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008014625-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10604742-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9498737-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9375499-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10316296-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109280662-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8993306-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014197107-A1 |
priorityDate |
2006-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |