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filingDate 2006-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21a56bc1e7c4258bda64abbdc178215a
publicationDate 2008-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2008076111-A1
titleOfInvention Methods and kits for determining sperm cell number
abstract A flow cytometry method and kit is provided for quantifying an absolute number of mature sperm cells in a semen sample, the method comprising (a) determining a total particle number (T); (b) determining a number of DNA comprising particles (D); (c) determining a number of cells which are not mature sperm cells (B) and; (d) determining a background signal (A); wherein steps (a) to (d) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal to [(T×D)/100]−(A+B), thereby quantifying the absolute number of mature sperm cells in the semen sample.
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