| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da90def4dcb4567ccc802c14d8a67d4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_26c7bbf63c9de2314ccfbd1639bc857b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate |
2007-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90e23793667e1934e842ec208d503021
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4987b20e49dcf3a28e242b7d1bc1c96 |
| publicationDate |
2008-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2008058221-A1 |
| titleOfInvention |
Methods for culture and production of single cell populations of human embryonic stem cells |
| abstract |
We used Accutase™, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8877493-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10246680-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012220031-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102597217-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015275179-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10316293-B2 |
| priorityDate |
2006-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |