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filingDate 2006-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3bc898235011afdf85fafe8f545359b
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publicationDate 2007-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2007275372-A1
titleOfInvention Methods and reagents for molecular detection of HIV-1 groups M, N and O
abstract Reagents and assays for detecting HIV-1 groups M and O and optionally HIV-1 group N and SIVcpz are provided. The reagents are nucleic acid primers for the hybridization to, amplification and subsequent detection of HIV-1 groups M, N and O and SIVcpz in a biological sample. The primers are oligonucleotides that selectively hybridize to the highly conserved regions of the env and pol regions of HIV-1. The assays employ the primers for HIV-1 nucleic acid amplification using amplification techniques such as reverse transcription and the polymerase chain reaction. The assays are useful for diagnosing an HIV-1 group M, HIV-1 group N, or an HIV-1 group O infection in a patient. Due to the high sensitivity of the assays, small concentrations of HIV in a biological sample can be detected, thereby allowing diagnosis at an early stage of infection. The assays are also useful for detecting HIV-1 contamination in a biological fluid such as blood. The assays are qualitative or quantitative and are therefore useful for viral load determinations of HIV-1 groups M, N or O in a patient undergoing treatment for HIV-1 infection. Viral load determinations can be used to monitor the progress of the treatment regimen or the development of drug resistance and can be used to predict disease progression.
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