| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0fad3ca58e8607ca9c1a9356d6816ba4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04994b7a9d93c8287399a926df5cc7e0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_06c23692a638c28b9b966bd890c7a3f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0695cdb49f4049c7fc69a9f01acab836
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a66f3e1d22f7b9674f58848550bc86c6 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate |
2004-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed7b24d76362592ed02e7e0e2ec2a895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd96500258e3e9467d3c623705328524
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24a06e87d860e1713993e84cec448792
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5ecfec845a13cf1d86d3c9789858bab |
| publicationDate |
2007-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2007087325-A1 |
| titleOfInvention |
Method of screening for cell colonies that express a soluble variant of a target protein |
| abstract |
The present invention concerns a method of identifying a cell colony which expresses a soluble variant of a target protein which method comprises (a) subjecting the cell colony to conditions which are capable of causing lysis thereof; (b) filtering the lysate through a filter having pores which allow only soluble proteins to pass through the filter; and (c) detecting target protein which has passed through the filter where the target is not detected an the basis of its own enzymatic activity. The invention further covers the identification of a cell colony expressing a soluble variant of a membrane protein using steps (a) and (b) of the method above. A kit for use in the methods is also covered by the invention. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012135535-A1 |
| priorityDate |
2003-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |