| abstract |
A cellular RNA isolation method comprising treating at least one nucleated cell with a composition comprising mild detergents such as non-ionic detergent(s), buffer(s), chelator(s), reducing agent(s), salt(s), and RNase inhibitor(s) to result in a cytoplasmic lysate and intact nucleus, then separating the cytoplasmic lysate containing RNA from the nucleus, and using the cytoplasmic lysate for RNA analysis without further purifying the RNA. |