| abstract |
The present teachings provide methods for diagnosing biological conditions, including cancer. In some embodiments, a test sample is collected from a subject such as a clinical patient, wherein the test sample comprises background tissue and may or may not contain cells from a tissue of interest. Observation of a target miRNA normally present in a tissue of interest, but collected in an anatomical location ectopic to the tissue of interest, can be indicative of a biological condition. The present teachings further provide exponential amplification techniques applicable to performing these analyses. |