| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9358ee7c06a9463adfe0c9e8ab6c2257 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-03048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y10-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65522
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65586
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-04 |
| filingDate |
2006-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1011641a02821a0d2ce47b1c695f2547
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5386a64d2cf9ce29169cbf73684a4e73 |
| publicationDate |
2007-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2007031892-A1 |
| titleOfInvention |
Novel coumarin-based amino acids for use in enzyme activity and substrate specificity assays |
| abstract |
The design, synthesis, and utilization of a novel coumarin-based amino acid and its fluorinated derivatives for use in enzyme activity and substrate specificity assays are described. The synthesis is both facile and high-yielding and allows for the production of a highly fluorescent phosphorylated amino acid that can be incorporated into peptides using standard SPPS techniques. The resultant substrates are efficiently hydrolyzed by PTPs and exhibit a large increase in fluorescence upon hydrolysis. Fluorinated CAP derivatives have utility as fluorogenic enzyme substrates. CAP and its derivatives can be incorporated into combinatorial peptide libraries for assaying enzyme substrate specificities. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010086957-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8399213-B2 |
| priorityDate |
2005-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |