| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5f811c62d6dfb4627e8b16461b4d5fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e6b8a9f8f3aa816951d86443a5ea9d0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8283
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 |
| filingDate |
2006-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_574a1336b62ee042697f204db1101076
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23e52248ca0796fe6361219b2307c7bd |
| publicationDate |
2006-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2006275830-A1 |
| titleOfInvention |
Isolation of Proteins Involved in Posttranscriptional Gene Silencing and Methods of Use |
| abstract |
The present invention includes a method for detecting and isolating sugarcane proteins that interact with the HC-Pro and P1 proteins of SrMV and other proteins involved in gene silencing, particularly in sugarcane. The method uses a two hybrid assay with an HC-Pro, P1, or other silencing-related protein-containing bait protein and a prey protein containing a polypeptide encoded by a DNA molecule in a cDNA library. The method also includes identification of false positives through reverse two-hybrid assays and using in vitro techniques such as farwestern blots or pull down assays where plant physiological conditions may be replicated. Finally, interactions may be confirmed in planta. Some novel proteins used in and discovered using the these methods are also identified. Methods of using viral and plant proteins to regulate silencing in plants such as sugarcane are also discussed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006272052-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7534614-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7632936-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7632937-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7777096-B2 |
| priorityDate |
2001-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |