| abstract |
Various embodiments of the present invention are directed to kinase-directed, activity-based probes (“KABPs”) that tightly bind to, and label, kinases. Each KABP includes a binding group that is recognized and bound by one or more kinases, a reactive group that tightly, and generally irreversibly, binds to the kinase, a tag group that labels the kinase, or that serves a chemical handle for subsequent procedures and processes, and a linker group that links the tag group to one or more of the reactive group and the binding group. Additional embodiments of the present invention are directed to methods for identifying kinases within, and isolating kinases from, living cells by use of one or more KABPs. |