| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e1fc53f7e08ce36e82cdda9ddbf31a07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_612eb61891a9a22167bd956152bf0e85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d0972bcee9bde715cce9b3fe64766ce
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f1e5d6003036d57e4f7fe9e6134be4b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1aaa774c9f10972de017e21300946d7e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90b125b5250554beabf7a198c8c9c5f0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2006-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_115106f1c569930ab82938134ec80837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_168ff89e22e2968907a47fe29549d145
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7da133a2843bbdb0247d6d68c23fb46f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92ed80c4db7314ca0e4dfd42fadeb5a7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7d6df8840fd0623ec08874144a55709
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2a3fe7b6b6db018d8df006b9ee71cc2 |
| publicationDate |
2006-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2006211010-A1 |
| titleOfInvention |
Labeled nucleotide phosphate (NP) probes |
| abstract |
The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site. A plurality of labelled types of nucleotide analogs are provided proximate to the active site, with each distinguishable type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid sequence. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand at the active site, where the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid at the active site. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The steps of providing labelled nucleotide analogs, polymerizing the growing nucleic acid strand, and identifying the added nucleotide analog are repeated so that the nucleic acid strand is further extended and the sequence of the target nucleic acid is determined. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11098356-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9243284-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8314216-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172868-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172861-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172858-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172863-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8648179-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008091005-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9797879-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008153095-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10408814-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7659070-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2016172215-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7668697-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008241938-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008241951-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11214832-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007275395-A1 |
| priorityDate |
1999-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |