| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_802253cc4f62e36b72dc07043bbaf355
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a5cbf5fef5bde453b3c7846439fca0d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b225a8d4fc2ccf5455635e9bf3208ab |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07J17-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| filingDate |
2005-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5e9527f702dca1fa15c4f71c4a35539
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b15cad61ac761b83b262e358f9482985
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95c6f40037a16e10a1797cea17da2994 |
| publicationDate |
2006-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2006177818-A1 |
| titleOfInvention |
Method of detection of classical swine fever |
| abstract |
The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5′-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5′ NTR was constructed and tested. Furthermore, a primer-probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013140339-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006286551-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111471803-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113999939-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7700326-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102244778-A |
| priorityDate |
2005-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |