| abstract |
The invention relates to a method of minimizing off-target effects of siRNA, comprising preparing double-stranded RNA (dsRNA) having a sense strand that is homologous to a sequence of a target gene and an anti-sense strand that is complementary to said sense strand, and having at least one pyrimidine replaced by a 5′-methyl-pyrimidine, and contacting said dsRNA with a cell capable of expressing said target gene. |