| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f38ea061e77ebf2cd140d25a1069c5a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b59b308d34d1d81ab614dbded84cde28 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2003-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c62f47243dafe79df17bf7af96bc9650
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5221a705e577dc1bba8b36c0ce8559ec |
| publicationDate |
2006-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2006094009-A1 |
| titleOfInvention |
Method for the characterisation of nucleic acid molecules |
| abstract |
A method for characterising nucleic acid molecules comprises the steps of i) introducing a modified base which is a substrate for a DNA endonuclease, for example Endonuclease V from E. coli , into a target DNA molecule; and ii) reacting the nucleic acid containing the modified base with said DNA endonuclease such that the nucleic acid is cleaved to generate an upstream fragment condition said modified base and bearing a 3′ hydroxyl group. The upstream fragment generated in step ii) can be used as a primer for a subsequent extension reaction. The modified base as such targets cleavage of the nucleic acid strand because it directs the endonuclease to cut the nucleic acid strand on which it resides at a site predominantly one nucleotide removed therefrom, so that the modified base remains in the nucleci acid as th upstream fragment. The method can be used for detecting polymorphisms and mutations and for nucleic acid indentification and profiling. The method enables one to detect polymorphisms in any sequence context and such detection is not dependent on surrounding nucleotide sequence. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8598088-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010030716-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011003700-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8343738-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010286379-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010041013-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011136098-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9732375-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012159060-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020154512-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010221785-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8431347-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8685675-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011171649-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010304386-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/TW-I465572-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010285537-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101136008-B1 |
| priorityDate |
2002-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |