http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006068396-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fc06cad2b58bbfcc30e3c96a6eb6c926 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2004-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6538e42c5144e3f2a11af3858b434aef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5816c56b1eb5b3f8943388e685eb199 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4a6c875d4d1141b65a6f0c273bc2d77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ccf207878ab2e73cca7c9ec79b3cd21 |
publicationDate | 2006-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2006068396-A1 |
titleOfInvention | Method for biochip detecting limited cells |
abstract | The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon membrane chip by a rapid nucleic acid cross-linker when preparing a chip; collecting some normal whole blood to be linearly amplified; synthesizing required amount of cDNA by a reverse transcription to obtain a marker as a probe; processing labeling, hybridization and post-hybridization to the chip and the marker; processing chemical color reaction; and automatically analyzing the result image after the chemical color reaction. Accordingly, a gene biochip operation technology platform with low cost, easy operation and high efficiency is obtained. And so the functions and the applications of the gene biochip can be effectively worked out and the practical applications of the gene biochip on related fields can be conclusively popularized. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011281774-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8178343-B2 |
priorityDate | 2004-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.