| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8b44caeacd03f5c1ef6df47bf94cd006 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14111
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14151 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 |
| filingDate |
2005-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9e154a4db54fa65fb2fe28d1cc53a9d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be76edbc243776cbbbf1eb26e54935bd |
| publicationDate |
2006-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2006035364-A1 |
| titleOfInvention |
Compositions and methods to prevent AAV vector aggregation |
| abstract |
Compositions and methods are provided for preparation of concentrated stock solutions of AAV virions without aggregation. Formulations for AAV preparation and storage are high ionic strength solutions (e.g. μ˜500 mM) that are nonetheless isotonic with the intended target tissue. This combination of high ionic strength and modest osmolarity is achieved using salts of high valency, such as sodium citrate. AAV stock solutions up to 6.4×10 13 vg/mL are possible using the formulations of the invention, with no aggregation being observed even after ten freeze-thaw cycles. The surfactant Pluronic® F68 may be added at 0.001% to prevent losses of virions to surfaces during handling. Virion preparations can also be treated with nucleases to eliminate small nucleic acid strands on virions surfaces that exacerbate aggregation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-4017986-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2470877-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022340895-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9862936-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9879229-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2011209743-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011094198-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013072548-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016185153-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014004568-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9408904-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102947453-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20160019477-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10328145-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105838737-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10080793-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014204303-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9644187-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102219672-B1 |
| priorityDate |
2004-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |