http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006030052-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f045c573da2dae6d1c779596c71c20ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76ad664c5c55e994c3b2d23eae75b756 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72a22594cd028f3d75d6632d6c79a050 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d85032c5056ac262c9319bc977e19b6 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6821 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00 |
filingDate | 2003-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_add46a77e20bca93ae5fe6a0b9acad68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1b89fba410164e0ae090b2fa365b5f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8777a74f02fed36cd3ba5be6021c847a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32b59e663ff11b4e256ab91de2d2c0d7 |
publicationDate | 2006-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2006030052-A1 |
titleOfInvention | Method of analyzing c-terminal amino acid sequence of peptide |
abstract | The present invention provides, as a method of analyzing the C-terminal amino acid sequence of a peptide with use of reaction technique for successively releasing the C-terminal amino acids, in which undesirable side reactions, such as cleavage of a peptide bond at the middle of the peptide, can be prevented and chemical treatments therein can be carried out under widely applicable conditions in the course of successive release of the C-terminal amino acids from a peptide, such a method comprising steps of dehydrating the gel on which a target peptide that has been separated by gel electrophoresis is held in the bound state; immersing it in a mixture solution of an alkanoic acid anhydride added with a small amount of a perfluoroalkanoic acid in a dipolar aprotic solvent to re-swell the gel carrier, forming a 5-oxazolone structure, at a temperature chosen in the range of from 30° C. to 80° C., followed by the cleavage of the 5-oxazolone ring to release the C-terminal amino acids, and then specifying the C-terminal amino acid sequence of the peptide based on the measured decrease in the molecular weight of a series of reaction products resulting therefrom. |
priorityDate | 2002-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 293.