Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d5d546aa6cbfb52c137af7cafc36bf4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y108-01008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 |
filingDate |
2004-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7155db8826288e74e50c30bbf5700e44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c32f3de6fc042d1c5ecc05ff428cefce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83f31372f8389a36ace712d1ba970185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adcd69a806aab9d1d7ee40be4b56a717 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bc89bcfdd6a0497d27d7bd362683bdd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1bcd4a0da7deb5ac87f4d9947612770d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65b4baffd22203e9690748e536ab0819 |
publicationDate |
2005-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2005255542-A1 |
titleOfInvention |
Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector |
abstract |
A fusion protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the fusion protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active three dimensional structure. Also, provided a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012088269-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11618777-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7745171-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008221311-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9005920-B2 |
priorityDate |
2002-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |