| abstract |
The present invention is related to a method for the construction of micro-arrays of polynucleotide sets ( 3 ) on a surface ( 5 ) of a solid support to be used for the detection and/or the quantification of at least four different target polynucleotides or nucleotide sequences ( 4 ), possibly present in a biological sample or test solution, said method comprising the steps of fixing nucleotide line sequences ( 1 ) upon a surface ( 5 ) of the solid support, said nucleotide lines ( 1 ) being at least 20 nucleotides long and having a random nucleotide sequence, deposing in at least 4 specific locations ( 6 ) on the surface ( 5 ) of the comprising said fixed nucleotide line sequences ( 1 ), solid support, nucleotide hooks ( 2 ) having a sequence specific for one of said at least four different target polynucleotides sequences ( 4 ) to be detected and/or quantified and covalently linking at said specific surface location ( 6 ) the nucleotide hooks ( 2 ) to the nucleotide lines sequence ( 1 ) in order to form polynucleotide sets ( 3 ), specific for the binding of said target polynucleotide sequence. |