| abstract |
A method for single nucleotide polymorphism (SNP) genotyping using widely available DNA sequencers is provided. A restriction endonuclease recognition site is incorporated into a PCR primer for the SNP, a restriction enzyme is used to cleave the DNA and create extendable ends at target polymorphic sites, and an extension reaction is used to create allele-specific extension products that can be distinguished using DNA sequencers or other detection platforms. |